On/Off Switches for Nitric Oxide Synthases

60% in golgi!

Dr. Venema:
�I did not realize that 60% of the eNOS was in golgi. �Can you post or send me the reference if this work is already published?
Thanx
akg

On Fri Dec 11, Richard C. Venema wrote
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>On Tue Dec 8, grover wrote
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>>Dr. Venema: �These are very interesting results. �Do you think that there are ways to isolate these pools of endothelial cells which respond differently to various situations to study their properties?
>>On Tue Dec 8, Richard C. Venema wrote
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>>>On Fri Dec 4, grover wrote
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>>>>Dr. Venema: Great presentation. �Hope you have fun at the meeting. �With so many different regulators of eNOS - I wonder if there is a hierarachy for regulation at different sites.
>>>>Dr. Grover: �I believe there are different pools of eNOS in endothelial cells that are activated in different ways by different combinations of allosteric effectors. �This may explain why NO release in response to Ca2+ ionophore is much greater than that in response to bradykinin or other hormones. �NO release in response to shear stress is even greater still and can be up to 20 times greater than in response to a hormone agonist.
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>>Dr. Grover: �I believe there are different pools of eNOS within the same cell. �We have found that eNOS is 10% cytosolic, 30% in caveolae, and 60% in Golgi. �These compartments can be separated by centrifugation on sucrose density gradients. �However, further subfractionation would be difficult.
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Fri Dec 11