Molecular Mechanisms of Ageing

Thanx

Dr. Toda
Thanx for the response.
akg
On Thu Dec 10, Tosifusa Toda wrote
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>On Fri Dec 4, grover wrote
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>>Dr. Toda:Hope you are enjoying the meeting. �Great poster. I am impressed. �A couple of related questions. �First, there are a number of proteins which have been identified with cell scenescence. �From your study of the data base, are those protein bands changing as well. � Essentially, I am wondering about verification with the current literature. �Second question. �Have you compared or have plans to compare similar data bases of different cells? �It is only then, we will find the common denominators. �I must say it is a powerful tool.
>>

>Dr. Grover,
>Thank you for your comment and question.
>Identification of spot proteins by mass spectrometry is now ongoing in our laboratory. But we have not yet succeeded in assigning any spot on the 2-D gel map to proteins which have been reported as a cell senescence marker. �We may �achieve direct identification of spot proteins if the specific antibody is available. But unfortunately we don't have such antibody. So I would like to ask for collaboration with researchers who may offer specific antibodies against cellular senescence-related proteins to assign the location on our 2-D gel map.
>We have a plan to compare our 2-D gel map with keratinocyte database prepared by Celis in Danish Centre of Human Genome Research. Common denominators will be applied to matched proteins between two databases.

>Tosifusa Toda
>

Fri Dec 11